Recombinant Enterokinase CAS 9014-74-8 Enteropeptidase

• Customization: Available
• CAS No.: 9014-74-8
• Formula: /
• Model NO.: YB223
• EINECS: 232-761-1
• Environmental Protection: No
• Certification: ISO9001
• Color: White
• Classification: Coenzyme
• Appearance: Salt-Free, Lyophilized Powd
• Type: Pharmaceutical Intermediates
• Quality: Refined
• Colour: White
• Samples: Available
• Certificates: ISO9001/SGS
• Package: 200u
• Stocks: in Stock
• Lead Time: 5-7 Working Days
• Shelf Life: 2-3 Years
• Site Audit: Available
• Transport: Air/Express/Sea
• MOQ: 1 Unit
• Payment Method: LC/Tt/DAP/OA
• Form: Lyophilized Powd
• Transport Package: Drum/Bottle/Bags
• Specification: 100units/mg protein
• Trademark: Yunbang OEM
• Origin: China

Product Description

Recombinant Enterokinase CAS 9014-74-8 Enteropeptidase

 

Company Informations

Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.

The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".

We can provide customers with customized processing, research and development services;
We can develop high-end Pharmaceutical and chemical products with high technical difficulties,high barriers and unique production processes;
We can provide 100 grams Grade, kilograms, and hundred kilograms of raw material pharmaceutical intermediates and other products.
We can perform -100°C to 300°C reaction.

R&D strength is the company's core competitiveness.Yunbangpharm has maintains a good cooperation relationship with many Universities and Research institutes locally, which can quickly transform R&D results into industrial production.

Our goal : To achieve the integrated upgrade of Pharmaceutical intermediates, raw materials and high-end chemical products, adhere to "serving pharmaceutical companies, creating our brands" to provide customers with high-quality and low-cost pharmaceutical and chemical products, realizing Win-win cooperation and common development.
 

Product Description

Application

Enterokinase from bovine intestine has been used to remove S-Tag and N-terminal His-tag from recombinant glutathione peroxidase 1 (Gpx1).[2] It has also been used to cleave interleukin-2 (IL-2) protein from the granules[3]

Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Biochem/physiol Actions

Enterokinase catalyzes the proteolytic activation of pancreatic. This action prevents the harmful tissue damage produced by the autoactivation of pancreatic in the pancreas. Enterokinase recognizes Lys or Arg residues in the peptide. Lack of enterokinase can harm food digestion and absorption mechanism.[1] Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.[2]

Packaging

Supplied with optimized enterokinase buffer

Unit Definition

One unit is that amount of enterokinase which results in >95% cleavage of 1 µg of purified FLAG-BAP fusion protein in 18 hours at 37 °C. One FLAG-BAP unit is equal to 10x the activity of a standard trypsinogen unit.

Other Notes

Do not used PVDF since free FLAG peptide will bind to the PVDF membrane.

FAQ

1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.

2: What's your MOQ?
A: We can have a discussion.

3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.

4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.

5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.

6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.

Our Advantages

1. 90days quality guarantee for exchange.

2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.

3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
 

Product analysis

Product Name:	ENTEROKINASE
Synonyms:	ENTEROPEPTIDASE;ENTEROPEPTIDASE, LIGHT CHAIN;ENTEROKINASE;ENTEROKINASE, LIGHT CHAIN;EC 3.4.21.9;enterokinase from porcine intestine;Peptidase, entero-;ENTEROKINASE, BOVINE
CAS:	9014-74-8
MF:	NULL
MW:	0
EINECS:	232-761-1
Product Categories:
Mol File:	Mol File

ENTEROKINASE Chemical Properties

storage temp.	-20°C
form	salt-free, lyophilized powder
color	white
CAS DataBase Reference	9014-74-8
EPA Substance Registry System	Peptidase, entero- (9014-74-8)

Safety Information

WGK Germany

3

 

ENTEROKINASE Usage And Synthesis

Uses	Typical conditions for fusion protein cleavage: Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween^? 20 Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix Incubate reaction mixture at ~25 °C for 16 hours
Definition	An enzyme found in the small intestine, which converts trypsinogen into trypsin.
General Description	Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.
Biochem/physiol Actions	Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

Detailed Photos