Customization: | Available |
---|---|
CAS No.: | 150-25-4 |
Formula: | C6h13no4 |
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Bicine Chemical Properties |
Melting point | 190 °C (dec.)(lit.) |
Boiling point | 290.25°C (rough estimate) |
density | 1.05 g/mL at 20 °C |
refractive index | 1.4240 (estimate) |
storage temp. | room temp |
solubility | H2O: 1 M at 20 °C, clear, colorless |
form | Powder |
color | White |
PH | 4.0-5.5 (25ºC, 1M in H2O) |
PH Range | 7.6 - 9.0 |
pka | 8.3(at 25ºC) |
Water Solubility | soluble |
λmax | λ: 260 nm Amax: 0.070 λ: 280 nm Amax: 0.040 |
Merck | 14,1201 |
BRN | 1769362 |
CAS DataBase Reference | 150-25-4(CAS DataBase Reference) |
EPA Substance Registry System | Bicine (150-25-4) |
Safety Information |
Hazard Codes | Xn |
Risk Statements | 20/21/22-36/37/38 |
Safety Statements | 24/25-36-26 |
WGK Germany | 3 |
RTECS | MB9700000 |
F | 3-10 |
TSCA | Yes |
HS Code | 29225000 |
MSDS Information |
Provider | Language |
---|---|
N,N-Bis(2-hydroxyethyl)glycine | English |
SigmaAldrich | English |
ACROS | English |
ALFA | English |
Bicine Usage And Synthesis |
Chemical Properties | White/clear crystalline powder |
Uses | To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM. |
Uses | Biological buffer and chelating agent. |
Purification Methods | Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals - Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.] |