| Customization: | Available |
|---|---|
| CAS No.: | 9001-99-4 |
| Formula: | / |
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Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.
The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".Ribonuclease A, derived from the bovine pancreas, is an endoribonuclease enzyme that cleaves RNA strands. This enzyme is known for its high specificity towards single-stranded RNA, cleaving the phosphodiester bonds between the 3' phosphate of one ribonucleotide and the 5' hydroxyl of an adjacent ribonucleotide.
Ribonuclease A has been extensively studied and utilized in various biochemical and molecular biology applications. It has been used in research for RNA degradation studies, RNA purification, and in the removal of RNA contamination from DNA samples. Additionally, it has been employed in molecular biology techniques such as RNA sequencing and in the preparation of RNA fragments for various experimental procedures.
The enzyme's ability to specifically cleave RNA molecules has also made it a valuable tool in structural biology, as well as in the development of therapeutic applications targeting RNA-based diseases.
In summary, Ribonuclease A from bovine pancreas is a widely studied and utilized enzyme with significant applications in biochemical research, molecular biology, and biotechnology, owing to its specific endoribonuclease activity.
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.
4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.
5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
1. 90days quality guarantee for exchange.
2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.
3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
| Product Name: | Ribonuclease A |
| CAS: | 9001-99-4 |
| MF: | NULL |
| MW: | 0 |
| EINECS: | 232-646-6 |
| Product Categories: | General Metabolic Enzymes;Metabolomics;Nucleotide Metabolism;3.x.x.x Hydrolases;Others;Enzyme Class Index;Nucleic acids;Ribonuclease A |
| Mol File: | Mol File |
| Melting point | 243 °C (dec.) |
| alpha | D25 (per mg N) -0.47° (c = 5) |
| storage temp. | -20°C |
| solubility | Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride |
| form | solid |
| color | white |
| PH | pH : 8.0 |
| Water Solubility | Soluble in water. |
| Sensitive | Moisture Sensitive |
| EPA Substance Registry System | Nuclease, ribo- (9001-99-4) |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | RF0760000 |
| F | 10-21 |
| TSCA | Yes |
| HS Code | 35079090 |
| Chemical Properties | Lyophilized |
| Uses | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography. |
| Uses | Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas. |
| Uses | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins. |
| General Description | Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils. |
| Biochem/physiol Actions | Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. |
| Purification Methods | Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins. |
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