• Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
  • Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
  • Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
  • Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
  • Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
  • Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4

Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4

CAS No.: 9001-99-4
Formula: /
EINECS: 232-646-6
Environmental Protection: No
Certification: ISO9001
Color: White
Samples:
US$ 2/Bag 1 Bag(Min.Order)
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  • Overview
  • Company Informations
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  • Product analysis
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Overview

Basic Info.

Model NO.
YB144
Classification
Coenzyme
Function
Catalysis
Appearance
Powder
Type
Pharmaceutical Intermediates
Quality
Refined
Colour
White
Samples
Available
Certificates
ISO9001/SGS
Package
1kg/5kg/10kg/25kg
Stocks
in Stock
Lead Time
5-7 Working Days
Shelf Life
2-3 Years
Site Audit
Available
Transport
Air/Express/Sea
MOQ
1 Unit
Payment Method
LC/Tt/DAP/OA
Form
Powder
Purity
99%
Transport Package
Drum/Bottle/Bags
Specification
60u/mg
Trademark
Yunbang OEM
Origin
China

Product Description

Ribonuclease A from bovine pancreas powder CAS 9001-99-4
 
Company Informations

Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.

The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".

We can provide customers with customized processing, research and development services;
We can develop high-end Pharmaceutical and chemical products with high technical difficulties,high barriers and unique production processes;
We can provide 100 grams Grade, kilograms, and hundred kilograms of raw material pharmaceutical intermediates and other products.
We can perform -100°C to 300°C reaction.

R&D strength is the company's core competitiveness.Yunbangpharm has maintains a good cooperation relationship with many Universities and Research institutes locally, which can quickly transform R&D results into industrial production.


Our goal : To achieve the integrated upgrade of Pharmaceutical intermediates, raw materials and high-end chemical products, adhere to "serving pharmaceutical companies, creating our brands" to provide customers with high-quality and low-cost pharmaceutical and chemical products, realizing Win-win cooperation and common development.

 
Product Description

Ribonuclease A, derived from the bovine pancreas, is an endoribonuclease enzyme that cleaves RNA strands. This enzyme is known for its high specificity towards single-stranded RNA, cleaving the phosphodiester bonds between the 3' phosphate of one ribonucleotide and the 5' hydroxyl of an adjacent ribonucleotide.

Ribonuclease A has been extensively studied and utilized in various biochemical and molecular biology applications. It has been used in research for RNA degradation studies, RNA purification, and in the removal of RNA contamination from DNA samples. Additionally, it has been employed in molecular biology techniques such as RNA sequencing and in the preparation of RNA fragments for various experimental procedures.

The enzyme's ability to specifically cleave RNA molecules has also made it a valuable tool in structural biology, as well as in the development of therapeutic applications targeting RNA-based diseases.

In summary, Ribonuclease A from bovine pancreas is a widely studied and utilized enzyme with significant applications in biochemical research, molecular biology, and biotechnology, owing to its specific endoribonuclease activity.

General description

RNase A is a single chain polypeptide containing 124 amino acids, linked by four disulfide bridges. In contrast to RNase B, RNase A is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Application

  • RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
  • RNase A is also used in RNA sequence analysis and protection assays.
  • RNase A has been used as a tool for computer-aided drug design.
  • RNase A supports the analysis of RNA sequences.
  • RNase A hydrolyze RNA contained in protein samples.
  • Purification of DNA is supported by RNase A.
Ribonuclease A from bovine pancreas has been used:
  • for plasmid DNA purification
  • to digest and avoid staining of double-stranded RNA during staining of nuclei
  • in immunoperoxidase and immunofluorescence detection
  • to treat cells prior to flow cytometry
  • as a component of post-hybridization buffer for washing FFPE tissue sections post in-situ hybridization
  • to treat cells during the preparation of outer membrane protein (OMP) extracts
  • RNase protection assays
  • Removal of unspecifically bound RNA
  • Analysis of RNA sequences
  • Hydrolysis of RNA contained in protein samples

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
 
FAQ

1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.

2: What's your MOQ?
A: We can have a discussion.

3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.

4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.

5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.

6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.

Our Advantages

1. 90days quality guarantee for exchange.

2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.

3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.

 

Product analysis
Product Name: Ribonuclease A
CAS: 9001-99-4
MF: NULL
MW: 0
EINECS: 232-646-6
Product Categories: General Metabolic Enzymes;Metabolomics;Nucleotide Metabolism;3.x.x.x Hydrolases;Others;Enzyme Class Index;Nucleic acids;Ribonuclease A
Mol File: Mol File
 
Melting point  243 °C (dec.)
alpha  D25 (per mg N) -0.47° (c = 5)
storage temp.  -20°C
solubility  Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride
form  solid
color  white
PH pH : 8.0
Water Solubility  Soluble in water.
Sensitive  Moisture Sensitive
EPA Substance Registry System Nuclease, ribo- (9001-99-4)
Safety Statements  22-24/25
WGK Germany  3
RTECS  RF0760000
10-21
TSCA  Yes
HS Code  35079090
 
Chemical Properties Lyophilized
Uses Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.
Uses Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.
Uses Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.
General Description Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Biochem/physiol Actions Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Purification Methods Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.
 
Detailed Photos

 

Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4
Ribonuclease a From Bovine Pancreas Powder CAS 9001-99-4



 

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Registered Capital
10000000 RMB
Plant Area
>2000 square meters