Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.
The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".
We can provide customers with customized processing, research and development services;
We can develop high-end Pharmaceutical and chemical products with high technical difficulties,high barriers and unique production processes;
We can provide 100 grams Grade, kilograms, and hundred kilograms of raw material pharmaceutical intermediates and other products.
We can perform -100°C to 300°C reaction.
R&D strength is the company's core competitiveness.Yunbangpharm has maintains a good cooperation relationship with many Universities and Research institutes locally, which can quickly transform R&D results into industrial production.
Our goal : To achieve the integrated upgrade of Pharmaceutical intermediates, raw materials and high-end chemical products, adhere to "serving pharmaceutical companies, creating our brands" to provide customers with high-quality and low-cost pharmaceutical and chemical products, realizing Win-win cooperation and common development.
Product Description
Application
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.[1]
Biochem/physiol Actions
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate[2].
Unit Definition
One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.
Physical form
Lyophilized sulfate-free powder containing buffer salts as citrate and EDTA
Analysis Note
Protein determined by biuret
FAQ
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.
4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.
5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
Our Advantages
1. 90days quality guarantee for exchange.
2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.
3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
Product analysis
| Product Name: |
α-Glycerophosphate dehydrogenase |
| Synonyms: |
EC 1.1.1.8;GDH;GLYCEROL-3-PHOSPHATE DEHYDROGENASE;ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE I;ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE X;ALPHA-GDH;ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE;A-GLYCEROPHOSPHATE DEHYDROGENASE |
| CAS: |
9075-65-4 |
| MF: |
NULL |
| MW: |
0 |
| EINECS: |
232-982-3 |
| Product Categories: |
enzyme |
| Mol File: |
Mol File |
| |
| |
| α-Glycerophosphate dehydrogenase Chemical Properties |
| storage temp. |
-20°C |
| form |
suspension |
| color |
white |
| EPA Substance Registry System |
Dehydrogenase, glycerol phosphate (9075-65-4) |
| |
| α-Glycerophosphate dehydrogenase Usage And Synthesis |
| Uses |
α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:
- in the reaction mixture to measure the glycerol kinase activity
- to demonstrate compartmentalized enzymatic reactions where NADH is involved
- in the reaction mixture assay for L-fuculose-1-phosphate aldolase
|
| General Description |
α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase. |
| Biochem/physiol Actions |
α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) . This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism. |
| Purification Methods |
The dehydrogenase is recrystallised by adding (NH4)2SO4 to 0.45 saturation at pH 5.5 at 4oC, and the small amount of precipitate is removed, then a saturated solution of (NH4)2SO4 is added dropwise from time to time over several days in the cold room. The crystals are collected and recrystallised until they have maximum activity. The enzyme is stable in half-saturated (NH4)2SO4 for several weeks at 4o. The equilibrium [dihydroxyacetone][NADH][H+]/[G-3-P][NAD] is 1.0 x 10 -12M in Tris buffer at 25o. It uses NAD ten times more efficiently than NADP. The Km for G-3-P is 1.1 x 10 -4M, for NAD it is 3.8 x 10-4M and for dihydroxyacetone it is 4.6 x 10-4M in phosphate buffer pH 7.0 and at 23.3o. Dihydroxyacetone phosphate and fructose-1,6-diphosphate are inhibitors. [Branowski J Biol Chem 180 515 1949, The Enzymes 7 85 1963, Young & Pace Arch Biochem Biophys 75 125 1958, Walsh & Sallach Biochemistry 4 1076 1965.] |
){kind=link}