| Customization: | Available |
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| CAS No.: | 9003-98-9 |
| Formula: | / |
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Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.
The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".Deoxyribonuclease I, derived from the bovine pancreas, is an endonuclease enzyme that specifically cleaves phosphodiester linkages within double-stranded DNA, as well as single-stranded DNA. The enzyme hydrolyzes the DNA backbone, resulting in the generation of DNA fragments with 5'-phosphate and 3'-hydroxyl ends.
Deoxyribonuclease I has been widely utilized in molecular biology and biochemistry for various applications, including the removal of DNA from RNA preparations, the digestion of DNA in protein-DNA complexes, and the fragmentation of DNA for studies such as chromatin structure analysis.
Additionally, the enzyme has been employed in the development of therapeutic applications, particularly in the field of gene therapy and in the treatment of certain medical conditions associated with excessive DNA accumulation.
In summary, Deoxyribonuclease I from bovine pancreas is a valuable tool in molecular biology research and biotechnology, with diverse applications in DNA purification, chromatin studies, and potential therapeutic interventions. Its ability to specifically target and cleave DNA molecules has made it an essential component in numerous experimental and practical contexts.
General description
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.
4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.
5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
1. 90days quality guarantee for exchange.
2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.
3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
| Product Name: | Deoxyribonuclease |
| CAS: | 9003-98-9 |
| MF: | NULL |
| MW: | 0 |
| EINECS: | 232-667-0 |
| Product Categories: | enzyme;Modifying EnzymesApplication Index;Protein and Nucleic Acid IsolationEnzyme Class Index;3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Elisa Kit-plant ELISA Kit |
| Mol File: | Mol File |
| density | 100 g/mL at 20 °C |
| vapor pressure | 0.004Pa at 25ºC |
| storage temp. | -20°C |
| solubility | Soluble in 0.15 M sodium chloride (5.0mg/L) clear. |
| form | solution (clear, colorless) |
| color | white |
| Water Solubility | 125g/L at 25ºC |
| Merck | 13,2923 |
| LogP | -1.3 at 20ºC |
| EPA Substance Registry System | Nuclease, deoxyribo- (9003-98-9) |
| Safety Statements | 23-24/25-22 |
| WGK Germany | 3 |
| RTECS | RF0750000 |
| F | 10-21 |
| TSCA | Yes |
| HS Code | 35079090 |
| Uses | Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase. |
| Uses | Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples. |
| Uses | Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis |
| General Description | DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
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| Flammability and Explosibility | Not classified |
| Biochem/physiol Actions | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Clinical Use | DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I. Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy. |
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