CAS No 72-57-1 Trypan Blue Powder Dye Content 60 %

• Type: Pharmaceutical Intermediates
• Appearance: Powder
• Quality: Refined
• CAS No.: 72-57-1
• Formula: C34h24n6na4o14s4
• Einecs: 200-786-7
• Model NO.: YB097
• Colour: Dark Greenish-Brown
• Samples: Available
• Purity: 99%
• Certificates: ISO9001/SGS
• Package: 1kg/5kg/10kg/25kg
• Stocks: in Stock
• Lead Time: 5-7 Working Days
• Shelf Life: 2-3 Years
• Site Audit: Available
• Transport: Air/Express/Sea
• MOQ: 10g
• Transport Package: Drum/Bottle/Bags
• Specification: Dye content 60%
• Trademark: Yunbang OEM
• Origin: China

Product Description

CAS NO 72-57-1 Trypan Blue powder Dye content 60 %

Company Informations

Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.

The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".

We can provide customers with customized processing, research and development services;
We can develop high-end Pharmaceutical and chemical products with high technical difficulties,high barriers and unique production processes;
We can provide 100 grams Grade, kilograms, and hundred kilograms of raw material pharmaceutical intermediates and other products.
We can perform -100°C to 300°C reaction.

R&D strength is the company's core competitiveness.Yunbangpharm has maintains a good cooperation relationship with many Universities and Research institutes locally, which can quickly transform R&D results into industrial production.

Our goal : To achieve the integrated upgrade of Pharmaceutical intermediates, raw materials and high-end chemical products, adhere to "serving pharmaceutical companies, creating our brands" to provide customers with high-quality and low-cost pharmaceutical and chemical products, realizing Win-win cooperation and common development.

 

Product Description

General description

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:

• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
• to stain plant tissue in order to visualize cell death.

 

FAQ

1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.

2: What's your MOQ?
A: We can have a discussion.

3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.

4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.

5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.

6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
 

Our Advantages

1. 90days quality guarantee for exchange.

2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.

3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.

Product analysis

 

 

 

Product Name:	Direct Blue 14
Synonyms:	C.I.DirectBlue14;c.i.directblue14,tetrasodiumsalt;centralineblue3b;chloramiblau3b;chloramineblue;chloramineblue3b;chlorazolblue3b;congoblau3b
CAS:	72-57-1
MF:	C34H24N6Na4O14S4
MW:	960.81
EINECS:	200-786-7
Product Categories:	Dyes and Pigments;Organics;Hematopoietic Stem Cell In Vivo/In Vitro TrackingReagents and Supplements;Stains, Dyes and IndicatorsStains and Dyes;Cell Culture;Cell Viability and Proliferation;Hematopoietic Stem Cell Biology;In Vivo/In Vitro Tracking of Stem CellsStem Cell Biology;Stains&Dyes, A to;Stem Cell Biology;T-U-V
Mol File:	72-57-1.mol

Melting point	>300 °C (lit.)
density	1.007 g/mL at 20 °C
storage temp.	dry at room temperature
solubility	H2O: soluble10mg/mL
Colour Index	23850
form	Powder
color	Dark greenish-brown
PH	9.8 (10g/l, H2O, 20ºC)
Water Solubility	10 g/L (25 ºC)
Merck	14,9792
BRN	4360496
Stability:	Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference	72-57-1(CAS DataBase Reference)
IARC	2B (Vol. 8, Sup 7) 1987
EPA Substance Registry System	Trypan blue (72-57-1)

Hazard Codes	T,Xi
Risk Statements	45-41-37/38-36/37/38-22
Safety Statements	53-45-36/37/39-36-26
RIDADR	2811
WGK Germany	3
RTECS	QJ6475000
TSCA	Yes
HS Code	32041400
Hazardous Substances Data	72-57-1(Hazardous Substances Data)
Toxicity	LD100 i.v. in rats: 300 mg/kg (Anderson)

 

Chemical Properties	Trypan Blue is a dark Blue crystalline solid or powder.
Chemical Properties	blueish grey powder
Uses	Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.
Uses	Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.
Uses	Biological stain.
Preparation	3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.
Definition	ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid).
General Description	Bluish-gray to dark blue powder.
Air & Water Reactions	Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.
Reactivity Profile	Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.
Fire Hazard	Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.
Biochem/physiol Actions	Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
Safety Profile	Suspected carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. Poison by intraperitoneal, intravenous, and subcutaneous routes. Experimental teratogenic and reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx NazO, and SOx
Potential Exposure	Used in dyeing textiles; leather and paper; as a biological stain.
Shipping	UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.
Waste Disposal	Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal.

 

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