| CAS No.: | 9012-54-8 |
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| Formula: | / |
| EINECS: | 232-734-4 |
| Environmental Protection: | No |
| Certification: | ISO9001 |
| Color: | White |
| Samples: |
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| Customization: |
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Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.
The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".Cellulase is a class of enzymes that are capable of breaking down cellulose, which is the main structural component of plant cell walls. These enzymes are produced by a variety of organisms, including fungi, bacteria, and protozoans. Cellulase enzymes act by hydrolyzing the β-1,4-glycosidic linkages in cellulose, leading to the degradation of the complex cellulose structure into simpler sugars such as glucose.
The ability of cellulase to break down cellulose has made it valuable in various industrial processes, such as biofuel production, textile manufacturing, and food processing. In biofuel production, cellulase is used to convert cellulose from plant materials into fermentable sugars for ethanol production. In the textile industry, it is used to create stonewashed denim by breaking down cellulose fibers and giving the fabric a worn appearance. Additionally, cellulase is used in food processing to improve the extraction of juices from fruits and vegetables, as well as to enhance the digestibility of animal feed.
General description
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.
4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.
5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
1. 90days quality guarantee for exchange.
2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.
3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
| Product Name: | cellulase |
| Synonyms: | cellulasefromtrichodermalongibrachia-tum;Fungalcellulase;1,4-[1,3:1,4]-BETA-D-GLUCAN;1,4-[1,3:1,4]-BETA-D-GLUCAN 4-GLUCANO-HYDROLASE;IUB: 3.2.1.4;EC 3.2.1.4;EC: 3.2.1.4;CELLULASE |
| CAS: | 9012-54-8 |
| MF: | NULL |
| MW: | 0 |
| EINECS: | 232-734-4 |
| Product Categories: | Biochemistry;Enzyme;Glycohydrolase;Hydrolase;3.2.x.x Glycosidases;3.x.x.x Hydrolases;Application Index;Carbohydrate hydrolysis&PTM analysis;Carbohydrate HydrolysisEnzyme Class Index;9012-54-8 |
| Mol File: | Mol File |
| cellulase Chemical Properties |
| density | 1.2 g/mL at 25 °C |
| vapor pressure | 0.004Pa at 25ºC |
| storage temp. | 2-8°C |
| solubility | deionized water: soluble5.0mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).) |
| form | powder |
| color | white |
| Water Solubility | Soluble in cold water. |
| LogP | -1.3 at 20ºC |
| EPA Substance Registry System | Cellulase (9012-54-8) |
| Hazard Codes | Xn,Xi |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37-2-45-23 |
| WGK Germany | 1 |
| RTECS | FJ5375000 |
| F | 3-10 |
| TSCA | Yes |
| HS Code | 35079090 |
| Brief Introduction | Cellulase naturally occurs in plant tissues; wherein it acts as a reinforcing material. It is thick and viscous in its physical arateristics, which makes it ideal for adding to foods and beverages to make ingredients combine and congealtogether. Cellulase is found in all plants and is quite abundant. Actually, it is the most widespread organic compound and is found in every habitat around the world. Its abundance and ease of acquisition make cellulase an ideal additive to foods and drinks, because it is cheap and easy to extract. Cellulase, as a nutrient, belongs to the category of roughage or fiber. Its binding properties make it popular among large-scale food and drink industries, who use it produce fruit juice, sparkling waters, spirits and other alcoholic beverages. When combined with other enzymes, cellulase is applied to produce wine; here its role is to extract foreign and unwanted substances from grape skins. In less quantities, cellulase is added as a congealant to washing and cleaning agents. |
| Biological enzymes | Cellulase is a group of enzymes that catalyze the hydrolysis of cellulose to produce glucose and low polymerizing fibers, including three major components of dextran endonuclease, dextran exonuclease and cellobietase. It is not a single enzyme, but a synergistic multi-component enzyme system. Cellulase is an off-white powder or liquid. In 1906, Seilliere found the cellulase in the digestive solution of the snail. The cellulase hydrolyzes the β-1, 4-glucosidic linkages in the fibers to produce soluble polymers and D-glucose. Endocellulase breaks down internal bonds that it interrupts the cellulose's crystalline structure, causing the exposure of cellulose polysaccharide chains. Exocellulase continues the process of endocellulase by breaking down the ends of the particular chains into maximum 4 units. That's how disaccharides or tetrasaccharides like the cellobiose appear. Exocellulases were found in 2 forms: CBHII acts at the non-reducing cellulose end while the CBHI acts at the reducing end. Beta-glucosidase or cellobiase usually hydrolyses the above mentioned exocellulase and produces monosaccharides. The oxidative cellulase uses radical reactions like dehydrogenase and cellobiose to depolymerize cellulose. The activity of cellulase is commonly to split cellulose and convert it into beta-glucose. The symbiotic bacteria of herbivores produce this form of cellulase. Humans and other animals, besides ruminants, cannot generate this form of cellulase. Thus, they can split it only partially by using fermentation and they are not able to utilize the energy from the fibrous plants. According to the source of the enzyme, the relative molecular mass of the lowest 5000, up to 400000. The optimum pH is 4.0 to 5.0. The optimum temperature is 40~60 ºC. Some of the metal ions Mg2 +, Cl2 + and neutral salts can activate the enzyme, and some other metal ions Ag2 +, Cu2 +, Mn2 +, Hg2 + and dyes can inactivate the enzyme. The addition of cellulase to the wine can improve the saccharification effect and the liquor yield, and reduce the viscosity of the mash. Cellulase mainly comes from Aspergillus Niger and Trichoderma. Figure 1 the molecular structural formula of the cellulase |
| Category | Cellulase includes a variety of hydrolases, belonging to a class of complex enzymes. Generally cellulase is divided into three categories: 1. Endoglucanase, also known as endo-(1 → 4)-β-D-glucanase, endo-cellulase or (1 → 4)-β-D-glucan-1, 4-glucan hydrolase (EC 3.2.1.4), which catalyzes any sites within the cellulose amorphous region or the (1 → 4)-β-D-glucan chain on the surface of microfilament. 2. Exo-glucanase, also known as exo (1 → 4)-β-D-glucanase, extracellular cellulase or (1 → 4)-β-D-glucan disaccharide hydrolase (EC 3.2.1.91), it is capable to release the cellobiose (sometimes with a small amount of glucose) through enzymatic digestion on the non-reducing end of the β-D-glucan chain. 3. β-glucosidase, also known as cellobiose or β-D-glucoside glucohydrolase (EC 3.2.1.21), being able to hydrolyze cellobiose and water-soluble fiber dextrin into glucose. The mode of action of each of these is as follows: (1) Endo-p-glucanase, 1,4-ß-D-glucan glucanohydrolase, CMCase, Cx: "random" scission of cellulose chains yielding glucose and cello-oligo saccharides. (2) Exo-P-glucanase, 1,4-ß-D-glucan cellobiohydrolase, Avicelase, C1: exo-attack on the non-reducing end of cellulase with cellobiose as the primary structure. (3) ß-glucosidase, cellobiase: hydrolysis of cellobiose to glucose. Many kinds of cellulose degrading strains can produce a variety of isozymes. Organisms capable of degrading cellulose mainly include a large number of fungi, some bacteria and a few protozoa, but its degradation property depends on the polymerization and crystallinity of cellulose and the degree of binding with hemicellulose and lignin. Crystalline cellulose is highly resistant to the degradation of cellulase, and most of the glucan chains in microfilament are not accessible to the cellulase. Moreover, any hydrogen bonds that have been cleaved by endoglucanase are easy to reform due to the stabilizing trend of the dextran chain. The degradation of crystalline cellulose must be mediated through the synergistic interactions of both endoglucanase and exoglucanase. The exoglucanase removes the cellobiose from the newly created terminal formed after the action of the endoglucanase, thereby preventing the re-formation of the glycosidic bond. These two enzymes play a coherent and coordinating effect, but they are all inhibited by cellobiose, and the action of beta-glucanase is usually the rate-limiting step in cellulose degradation. Cellulase is a class of inducible enzymes and is repressed by catabolic products. Sophora is an excellent natural inducer of cellulase. Suppressors include glucose and other readily available substrates. Depending on the concentration, the cellobiose is either an inducer or a repressor. |
| Application in Detergent Industry | Fibrous fabrics, especially cotton fiber fabrics, often generate many microfiber fluffs after wearing and multiple laundering. These fluffs are wrapped together with organic and inorganic stains to form a lot of balls, resulting in darker and hardened surfaces of the clothes. To solve this problem, as early as 1970, it had been put forward in foreign patents of the idea of using fiber enzymes to eliminate these microfibers, but the idea had failed to become the reality for a long time. It was until 1985, used of the method of fermentation of Rhizopus sp. to make the world's first detergent-purpose cellulase. The product was named Cellulase. In 1987, a bacterial cellulase was introduced and successfully used in Attack detergent. Since then, cellulase also officially joined the ranks of detergent enzymes. Now, the application of cellulase in detergent is not too popular, but the brand-name products of some large companies have already used it. For example, the United States P & G some of the detergent, it contains this cellulase. Cellulase has different mechanism of action with other detergent enzyme. Instead of catalyzing the decomposition of a substance in the stain to make it become substance which can be washed with water to achieve the purpose of cleaning, cellulase takes effect through its micro-fiber effect on the fabric, achieving the purpose of finishing and renovation of the fabric. Because natural fibers, especially cotton fibers are glucose-composing macromolecules. The sugar in the molecule is only bound by β-1, 4-glucose to form a linear macromolecule. This kind of molecule was assembled into a bundle, known as the fibril. The assembly of a lot of fibrils leads to microfiber. Under normal circumstances, the fiber is arranged in a crystal way, so its surface is smooth, soft with bright appearance. However, after wearing, rubbing and repeated washing, some microfibers will be out of its crystallization area, forming many microfibers on the fiber surface or between fibers. These microfibers are wrapped around each other into pompons. They can not only make the clothes be dirty due to incorporation of the dirt substances, but also make both the single-color or color clothes become darkened because of the light scattering on the ball. If you want to remove these puffs from the clothing, then you need to choose the appropriate cellulase in order to complete this task well. In other words, only enzymes that meet the following conditions can be used in detergents. |
| Cellulose and Cellulase | Cellulose is a kind of macromolecule compound which is composed of many glucose molecules connected with each other. It is the most abundant polysaccharide-class natural organic compound on the earth. Cotton is the natural product of the highest cellulose content. In addition, wood, crop straw are also rich in cellulose, and many of the commercial cellulose are made from natural cellulose. Cellulose is a straight-chain glucose polymer connected with beta-1, 4 chains. The cellulose bundles are typically linked by hydrogen bonds to form a larger monomer. There are many different views on the number of cellulose molecules in such monomers and how they are organized. There is a view that some areas of a cellulosic molecule are ordered, structurally solid and non-flexible (crystalline cellulose), while other regions are flexible, string-like "wound" structure (amorphous state cellulose). When the cellulose fibers inhale of water, they will swell with the swelling effect being limited to the amorphous regions of the fiber while the crystalline area of the solid hydrogen bonding grid make them be free from swelling. The amount of bonds that the enzyme can act depends on the degree of swelling of the cellulose. In order to let the cellulase to efficiently hydrolyze the cellulose, it is usually necessary to pretreat to improve the swelling of the fiber. Cellulase is a complex enzyme. It is generally believed that it consists of at least three components, namely, C1 enzyme, CX enzyme and glucosidase. The first two enzymes can decompose cellulose into cellobiose while the third enzyme will break down the cellobiose into glucose. It is exactly under the synergistic effect of these three enzymes, cellulose is finally hydrolyzed into glucose, providing nutrition for microbial growth but also for human use. |
| Application | Cellulase has been widely used in textile, household chemicals, paper, food fermentation, industrial washing, tobacco, oil extraction, wastewater treatment and feed and other fields, its application prospects are very broad. Natural cellulases are found in microorganisms, certain insects and plants. Upon the germination of plant seed, cellulase hydrolyzes the seed coat cellulose, being conducive to germination. The symbiotic bacteria and protozoa in the gastrointestinal tract of the ruminant can secrete the cellulase and hydrolyze the cellulose into the sugar that can be absorbed. The use of microbial secretion of cellulase for agricultural and sideline products processing can produce high-quality feed and glucose. In Agriculture, people apply Trichoderma viride (a fungus being rich in cellulase) for feed fermentation, being able to improve the nutritional value of cellulose feed. Cellulase products are one of the main reagents for isolating plant protoplasts. In plant cell fusion experiments, it is often applied of cellulose or snailase to remove cell walls to isolate and obtain the desired protoplasts. Most industrial cellulases are glucosidase, containing a catalytic region and a cellulose linker. The cellulose would have a low activity for cellulose if it is removed of the cellulose attachment region. People have done a comparative study on cellulase and protease, finding that there is a great relationship between cellulase activity and the strength of adsorption. The 169th tyrosine residue in the catalytic region of cellobiose hydrolase from Trichoderma reesei can facilitate the conversion of the glucose ring to a more readily reactive configuration. (1) enzyme preparation; (2) mainly used for the softening and peeling of cereals, beans and other plant foods; (3) it is used for reducing the viscosity of coffee extract with the maximum allowable amount of 100mg/kg; (4) for the preparation of raw materials pretreatment; (5) for the extraction during the manufacture of defatted soy flour and isolated soy protein; (6) for the manufacture of starch, agar and algae food; (7) the elimination of turbidity caused by fiber contained in fruit juice, wine and beer; instant dissolving of green tea, black tea, etc., As a feed additive, it can help animals to digest and absorb the feed. It can hydrolyze theβ-1, 4-glucan contained in cellulose polysaccharide into β-dextrin. |
| Preparation | Use Trichoderma viride as raw material for fermentation, followed by fractional precipitation of ammonium sulfate with refinement to get the products. It is generally applied (Aspergillus Niger) or Trichoderma reesei for culturing, followed by salting out the fermentation broth for precipitation and refinement to obtain it. The resulting product contains not only cellulase, but also hemicellulase, pectinase, protease, lipase, xylanase, cellobiosease and amyloglucosidase. |
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