| Customization: | Available |
|---|---|
| Type: | Pharmaceutical Intermediates |
| Appearance: | Powder |
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| Chemical Properties |
| solubility | H2O: 10 mg/mL, clear, colorless |
| storage temp. | -20°C Freezer |
| form | White solid |
| color | White to Off-White |
| Safety Information |
| Hazard Codes | Xi |
| WGK Germany | 3 |
| Usage And Synthesis | |
| General description | A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase. |
| Uses | A non detergent sulfobetaine used as a mild solubilizing and stabilizing agent for chicken egg white lysozyme and E. coli ?-galactosidase. At 1M, NDSB256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800mM it will restore 16% of the enzymatic activity of denatured ?-galactosidase. Reduces aggregation and significantly improves protein renaturation. Zwitterionic over a wide pH range. |
| Uses | A nondetergent sulfobetaines class member |
| Reconstitution | Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature. |
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