Hunan Yunbang Pharmaceutical Co., Ltd. (Yunbangpharm) located in Changsha High-tech Industrial Park, Hunan. It is a high-tech enterprise specializing in the research and development, production and sales of Pharmaceutical raw materials, Pharmaceutical intermediates(API) and Fine chemicals. Based in China, Yunbangpharm has been providing suitable solutions for many domestic,foreign pharmaceutical companies and traders. Excellent quality, good reputation and authentic price have won the praise of the majority of customers.
The company adheres to the corporate tenet of "We do not produce medicines, but help pharmaceutical companies make good medicines".
We can provide customers with customized processing, research and development services;
We can develop high-end Pharmaceutical and chemical products with high technical difficulties,high barriers and unique production processes;
We can provide 100 grams Grade, kilograms, and hundred kilograms of raw material pharmaceutical intermediates and other products.
We can perform -100°C to 300°C reaction.
R&D strength is the company's core competitiveness.Yunbangpharm has maintains a good cooperation relationship with many Universities and Research institutes locally, which can quickly transform R&D results into industrial production.
Our goal : To achieve the integrated upgrade of Pharmaceutical intermediates, raw materials and high-end chemical products, adhere to "serving pharmaceutical companies, creating our brands" to provide customers with high-quality and low-cost pharmaceutical and chemical products, realizing Win-win cooperation and common development.
Product Description
Antipain dihydrochloride is a synthetic compound derived from the polypeptide alkaloid antipain. It is a protease inhibitor that specifically targets various proteolytic enzymes such as trypsin, chymotrypsin, and papain. Antipain dihydrochloride is commonly used in biochemical and cell biology research to inhibit protease activity and prevent the degradation of proteins in cell and tissue samples.
General description
Antipain is a protease inhibitor isolated from actinomycetes. It inhibits thrombokinase and blood coagulation.[1]
Application
Concentrations for 50% inhibition (μg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM
Formula: C27H44N10O6 x 2 HCl
Preparation Note
Working concentration: 50 μg/ml
Working solution: Soluble in water, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, (CHCl3), or petroleum and ethyl ethers.
Storage conditions (working solution): -15 to -25 °C
Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Growth of microorganisms and repeated freezing should be avoided. Stable for approximately one month.
Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, A stock solution in water or buffer is stable for about a month at -20 °C.
Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Reconstitution
Soluble in water, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, (CHCl3), or petroleum and ethyl ethers. Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Stable for approximately one month.
CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc.
FAQ
1:Can I get some samples before bulk order?
A: Most products provide free samples, but the shipping cost be paid by customers.
2: What's your MOQ?
A: We can have a discussion.
3: Which kind of payment terms do you accept?
A: PI will be sent first after confirmation of order,enclosed our bank information.Payment by T/T, Western Union,D/P, ETC.
4.How to place an order?
A: You can contact me through Trademanager, WhatsApp, Email and other contact methods, tell me the product and quantity you need, and then we will give you a quote.
5:How about your delivery time?
A: Generally, it will take 3 to 5 days after receiving your advance payment.
6:How do you treat quality complaint?
A: First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.
Our Advantages
1. 90days quality guarantee for exchange.
2. For each batch order extracts, we will attach a factory Inspection Report with the goods delivery , including the BatchNo/ Date/ Test index ects; also will keep the archived extracts to ensure received by customers are the same each time.
3. Our team of experienced professionals are available online 24 hours a day to answer any questions and provide guidance on product usage and maintenance.
Product analysis
| Product Name: |
ANTIPAIN, DIHYDROCHLORIDE |
| Synonyms: |
ANTIPAIN;ANTIPAIN (2HCL);ANTIPAIN, DIHYDROCHLORIDE;ANTIPAIN HYDROCHLORIDE;[(S)-I-CARBOXY-2-PHENYLETHYL]-CARBAMOYL-L-ARG-L-VAL-ARGINAL;[(S)-1-CARBOXY-2-PHENETHYL]-CARBAMOYL-ARG-VAL-ARG-ALDEHYDE;(S)-[1-CARBOXY-2-PHENYL-ETHYL]-CARBAMOYL-ARG-VAL-ARG-ALDEHYDE;[(S)-1-CARBOXY-2-PHENYLETHYL]-CARBAMOYL-ARG-VAL-ARG-AL HYDROCHLORIDE |
| CAS: |
37682-72-7 |
| MF: |
C27H46Cl2N10O6 |
| MW: |
677.62 |
| EINECS: |
609-466-5 |
| Product Categories: |
peptides;inhibitor;AN-AZ;Antineoplastic and Immunosuppressive Antibiotics;Broad Spectrum Inhibitors of Proteolytic Enzyme Classes;Cathepsin D;Cysteine Protease Inhibitor;A to C;Aldehydes;Antibiotics;Antibiotics by Application;Apoptosis Enzymes;Application Index;Bioactive Small Molecules;Biochemicals and Reagents;Building Blocks;C13-C60;Calpain;Calpain II;Calpain II Inhibitors;Carbonyl Compounds;Cathepsin B;Cathepsin/Granzyme Inhibitors &;Cell Biology;Cell Signaling and Neuroscience;Cell Signaling Enzymes;Chemical Structure Class;Chemical Synthesis;Coagulation Proteins and Reagents;Core Bioreagents;Substrates;Pepetides |
| Mol File: |
37682-72-7.mol |
| |
| Melting point |
209-217 °C |
| Boiling point |
652.92°C (rough estimate) |
| density |
1.2423 (rough estimate) |
| refractive index |
1.6500 (estimate) |
| storage temp. |
-20°C |
| solubility |
H2O: 50 mg/mL |
| form |
White powder |
| color |
White |
| Water Solubility |
0.01M in water |
| Stability: |
Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month. |
| Safety Statements |
22-24/25 |
| WGK Germany |
3 |
| RTECS |
YV9350800 |
| F |
1-10 |
| HS Code |
2934999090 |
| Description |
Antipain is a protease inhibitor originally isolated from actinomycetes. It inhibits thrombokinase, plasmin, trypsin, and papain in vitro (IC50s = 20, 93, 0.26, and 0.16 μg/ml, respectively). Antipain (6-600 μg/ml) inhibits the morphological transformation of and increases frequency of chromosomal aberrations in Syrian hamster embryo cells induced by N-methyl-N''-nitro-N-nitroso-guanidine (MNNG). In vivo, antipain (25-100 mg/kg) suppresses urethan-induced formation of cleft palates and cleft lips in mice. |
| Uses |
Concentrations for 50% inhibition (μg/ml): papain, 0.16trypsin, 0.26cathepsin A, 1.19cathepsin B, 0.59cathepsin D, 125plasmin, >93chymotrypsin and pepsin, >250It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM |
| References |
1) Umezawa?et al.?(1976),?Structures and activities of protease inhibitors of microbial origin; Method Enzymol.,?45?678 2) Gotoh?et al.?(2001),?Proteolytic activity and recombinant protein production in virus-infected Sf-9 insect cell cultures supplemented with carboxyl and cysteine protease inhibitors; J. Biosci. Bioeng.,?92?248 3) Hockensmith?et al. (2016), Identification and characterization of a chymotrypsin-like serine protease from periodontal pathogen, Tannerella forsythia; Microb. Pathog.,?100?37 4) Mat Amin?et al. (2004),?Proteinases in Naegleria Fowleri (strain NF3), a pathogenic amoeba: a preliminary study.; Trop. Biomed.,?21?57 5) Moriyyasu & Inoue (2008),?Use of protease inhibitor for detecting autophagy in plants; Methods Enzymol.,?451?557 |
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